1. INTRODUCTION:

A regular structure with a repetitive topology imprinted by nature is a characteristic of zeolites, which are composed of silicon, phosphorus, aluminum, and oxygen atoms. Stilbite was discovered by Swedish mineralogist Axel Fredrik Cronsted in 1756, which is when "zeolites" was first used. Porous silicates of aluminum are known as zeolites. Structural zeolites are crystalline substances that are porous and possess a distinctive structure and framework. An analysis of the interconnected network of zeolites indicates that silicon or aluminum atoms are situated in the center of the four-sided network, while oxygen atoms are situated in its extremities.

There are two types of zeolites: natural and synthetic. Certain components are combined to create synthetic zeolites, which develop rapidly. There are a few ways to use hydrothermal (100–200°C) and alkaline conditions to rapidly produce zeolites with small or big crystalline sizes. However, compared to manufactured zeolites, natural zeolites, that are formed over millions of years from sea salt as well as activated volcano ash, have greater grate particle sizes. Scanning electron micrographs for a typical synthetic zeolite are explained in Figure 1.

Figure. 1. SEM of synthetic zeolites

A growing body of research has demonstrated the modified and tailored biological activity of zeolites in recent years. Researchers were able to demonstrate in 2014 that while humans bone marrow stromal cells (hBMSCs) grown on the surface of ZSM-5 zeolite adhered, anatomically expanded, as well as multiplied with time, the quantity of living cells increased dramatically. However, zeolites are well known for their ability to absorb dangerous substances and ions. Actually, all positively charged molecules and ions are more easily absorbed due to their crystalline structure, which is made up of tiny, interconnected negatively charged channels. The majority of dangerous chemicals with positive charges have the potential to become trapped in zeolite canals. Additionally, the radioactive material from the Chernobyl power plant accident is absorbed using this feature (Rodriguez-Fuentes et. al 1997). Nano zeolite A and Y kinds were produced by Thomassen et al. and ranged in size from 25 to 100 nm. The toxicity study showed no discernible toxicity for nano-zeolite at concentrations of 500 μg/ml (Baerlocher et.al 2007).

2. Zeolites' physical, chemical, and morphological properties:

Consequently, silicate tetrahedra structures are formed by the macromolecular three-dimensional structure of the tetrahedral construction materials SiO₂ and AlO₂. A basic structure found in all zeolite- Based materials is the aluminosilicate framework. According to Nejati-Koshki et al. (2017), the structure is arranged around the tetrahedral configuration of Si⁴⁺ as well as aluminum Al³⁺, which are made up of four anions of O²⁻. The elements of zeolite crystal formations are their major and secondary building blocks. The tetrahedra (AlO₄)⁵⁺ as well as (SiO₄)⁴⁺ are the main components. A unique arrangement with straightforward geometry is produced by sharing oxygen atoms with a nearby tetrahedral. Additionally, secondary construction blocks may consist of polyhedra, single rings, double rings, or intricate elements are combined with a variety of ways to create a unique cage and channel system that is suitable for specific industrial or therapeutic applications (Mohammadian et .al 2017).

3. Zeolites' applications:

Zeolite is one of the useful materials in various applications due to its diversity of structures, which include pore sizes ranging from 2 to 13A°, form variations with 4–12 loops, and inter-network connections (Sadegh et .al 2015). Zeolites are considered "magic stones" due to their extensive potential for a variety of biological, industrial, agricultural, commercial, and technological applications (Montazeriet .al 2017). Most synthesized zeolites have found application in the industrial sector. However, natural zeolites have supplanted synthetic ones and are paving the way for their expansion in the market due to their lower cost (Sadegh et .al 2015). In the past, natural zeolites were utilized as additives in the cement industry (Firouzi-Amandi et .al 2018 and Farajzadeh et .al 2018).

3.1. The use of zeolites in medication delivery systems:

The Zeolites can also be used as a part of systems and biosensors that collect and measure biomarkers of serious diseases, including cancer. Biosensing can be done with potentiometric biosensors made by co-immobilizing enzymes with types of zeolites that pass through pH-ion-sensitive field-effect transistors. Enzyme-based electrochemical biosensors using a wide range of zeolites have been developed. The biorecognition elements of the biosensors were integrated with the zeolites that acted either as enzyme adsorbents or as the additional bio membrane elements. The biorecognition parts of the biosensors contained zeolites, which acted either as an enzyme adsorbent or additional bio membrane parts. The high concern in the detection of urea in clinical and biological analysis with analysis resulted in the successful development of a zeolite-linked polymeric membrane biosensor (clinoptilolite). The conductometric determination of L-arginine is especially beneficial due to the nature of clinoptilolite. The biorecognition parts of the biosensors were supplemented with zeolites which were adsorbents of enzyme or additional bio membrane parts. Urease is covalently bonded to the surface of an ammonium-sensitive field-effect transistor (FET) to make a urea biosensor. L-arginine is an amino acid that is temporarily essential to the human health. Infants require it in their development stage and some pathological conditions in adults. This lays stress on the need to observe the occurrence of arginine-based drugs in physiological fluids and control their quality. Therefore, the occurrence of the production of L-arginine conductometric biosensors was forecasted on the cross-linking of urease and arginase with glutaraldehyde in a single selective membrane and the subsequent modification with clinoptilolite. Biosensors are advanced equipment, which has been employed to determine the composition of an array of different media such as blood, urine, and environmental samples. The electrode sensor was used to measure trypophan (Trp) and dopamine (DA) in submicromolar concentrations.

The use of organophosphate insecticides has had a dire impact on the ecosystem, human health and the environment as well. Consequently, it is of utmost importance to keep a check on these insecticides. Scientists have come up with better biosensors to monitor the presence of orga-phosphate pesticides in a quick, easy-going and online manner. In order to identify the pesticides that were more active than the conventional polyaniline, a polyanilinenano crystalline zeolite-based acetylcholinesterase biosensor was employed. Recently, glucose biosensors based on Escherichia coli have been demonstrated. Despite certain drawbacks, such as decreased electrode stability and glucose dehydrogenase (GDH) activity, the use of zeolite in biosensors has the potential to boost enzyme activity. SiO₂ substrates are usually chemically modified through silanization processes in order to assemble the required biological elements onto modified substrates which are used in the biosensor and electrical applications. Microfabrication and electron beam lithography (EBL) can be used to prepare decorations on zeolite nanoparticles on SiO₂. Three varying assembling methodologies were considered to achieve this goal. These are manual assembly processes, ultrasound-assisted vigorous agitation (US), and spin coating (SC). The Human Immunodeficiency infection (HIV) is a deadly infection that affects a considerable number of the population in the world. In the recent past, a zeolitic imidazolate based biosensor that is capable of detecting HIV-1 DNA has been developed. ZIF-8, which is a zeolitic imidazolate framework used as a quenching plat form in detecting HIV-1 DNA, has been demonstrated to be a very effective and very sensitive detection form. Graphene oxide nanocrystals (Zeo–GO) and zeolite nanoflakes were recently used to develop a paper-based device that measures ketamine electroanalysis. The apolyaniline zeolite nanocomposite material was designed to sense acetylcholine, a neurotransmitter, in a manner that is highly sensitive. The developed biosensor was able to detect dangerous organophosphate pesticides. Consequently, it is required to diagnose the disease at the earliest stage, determine the prognosis of treatment, evaluate the curative efficacy, and determine the survival of HCC lesions in the long term. Ion exchange was therefore used to produce silver enclosed EMT zeolite nanoparticles (NPs), which were later used to determine alpha-fetoprotein (AFP). The EMT-based immunosensors including silver contain silver have a sufficiently calibrated surface area and conductivity, and show outstanding results in the detection of AFP.

3.2. Using zeolites to make biosensors and detect biomarkers:

Urease is covalently bonded to the surface of an ammonium-sensitive field-effect transistor (FET) to make a urea biosensor. L-arginine is an amino acid that is temporarily essential to the human health. Infants require it in their development stage and some pathological conditions in adults. This lays stress on the need to observe the occurrence of arginine-based drugs in physiological fluids and control their quality. Therefore, the occurrence of the production of L-arginine conductometric biosensors was forecasted on the cross-linking of urease and arginase with glutaraldehyde in a single selective membrane and the subsequent modification with clinoptilolite. Biosensors are advanced equipment, which has been employed to determine the composition of an array of different media such as blood, urine, and environmental samples. The electrode sensor was used to measure trypophan (Trp) and dopamine (DA) in submicromolar concentrations. The use of organophosphate insecticides has had a dire impact on the ecosystem, human health and the environment as well. Consequently, it is of utmost importance to keep a check on these insecticides. Scientists have come up with better biosensors to monitor the presence of orga-phosphate pesticides in a quick, easy-going and online manner. A polyanilinenano crystalline zeolite-based acetylcholinesterase biosensor was used to detect the pesticides that were more active than the traditional polyaniline. Recently, glucose biosensors based on the Escherichia coli have been shown. The use of zeolite in biosensors has the potential to increase the enzyme activity despite some of its limitations like reduction in the stability of the electrodes and glucose dehydrogenase (GDH) activity.

SiO₂ substrates are usually chemically modified through silanization processes in order to assemble the required biological elements onto modified substrates which are used in the biosensor and electrical applications. Microfabrication and electron beam lithography (EBL) can be used to prepare decorations on zeolite nanoparticles on SiO₂. Three varying assembling methodologies were considered to achieve this goal. These are manual assembly processes, ultrasound-assisted vigorous agitation (US), and spin coating (SC). HIV is a deadly infection that affects a considerable number of the population in the world. In the recent past, a zeolitic imidazolate based biosensor that is capable of detecting HIV-1 DNA has been developed. ZIF-8, which is a zeolitic imidazolate framework used as a quenching plat form in detecting HIV-1 DNA, has been demonstrated to be a very effective and very sensitive detection form. Graphene oxide nanocrystals (Zeo–GO) and zeolite nanoflakes were recently used to develop a paper-based device that measures ketamine electroanalysis. The apolyaniline zeolite nanocomposite material was designed to sense acetylcholine, a neurotransmitter, in a manner that is highly sensitive. The developed biosensor was able to detect dangerous organophosphate pesticides. Consequently, it is required to diagnose the disease at the earliest stage, determine the prognosis of treatment, evaluate the curative efficacy, and determine the survival of HCC lesions in the long term. Ion exchange was therefore used to produce silver enclosed EMT zeolite nanoparticles (NPs), which were later used to determine alpha-fetoprotein (AFP). The EMT-based immunosensors including silver contain silver have a sufficiently calibrated surface area and conductivity, and show outstanding results in the detection of AFP.

4. CONCLUSION AND PROSPECTS FOR THE FUTURE:

Zeolites are highly absorbent, biocompatible, antibacterial, nutritive, and non-toxic substances that are finding growing use in a range of medical research fields. A good choice of natural or synthetic zeolites conjugated with polymers is essential for applications in gas absorption, hemodialysis, implant coating, tissue engineering scaffolds, drug delivery systems, wound healing, and the removal of harmful ions from the body. Applications, biological characteristics, physical characteristics, and chemical characteristics are used to categorize zeolite, which might aid researchers interested in utilizing it in choosing the right zeolite. The current analysis has shown that the most common application of zeolite in the medical sciences is in drug delivery systems. This is supported by research on the absorption and discharge of a diverse array of pharmaceuticals, including anticancer medications. The introduction of novel treatment classes whose biological activity is contingent upon intelligent delivery methods and the development of innovative zeolite-based delivery systems is the primary reason for the increasing utilization of zeolite in biomedical sciences. We expect that this trend will persist for an extended period.

5. REFERENCES:

1. Rodríguez-Fuentes, G., Barrios, M. A., Iraizoz, A., Perdomo, I., and Cedré, B. Zeolite Research and Scientific Papers on Clinoptilolite. Zeolites. 1997; 19(5-6): 441-448.

2. Baerlocher, C., McCusker, L. B., and Olson, D. H. (2007). Atlas of zeolite framework types. Elsevier.

3. Nejati-Koshki, K., Pilehvar-Soltanahmadi, Y., Alizadeh, E., Ebrahimi-Kalan, A., Mortazavi, Y., and Zarghami, N. Development of Emu oil-loaded PCL/collagen bioactive nanofibers for proliferation and stemness preservation of human adipose-derived stem cells: possible application in regenerative medicine. Drug Development and Industrial Pharmacy. 2017; 43(12): 1978-1988.

4. Mohammadian, F., Pilehvar-Soltanahmadi, Y., Zarghami, F., Akbarzadeh, A., and Zarghami, N. (). Upregulation of miR-9 and Let-7a by nanoencapsulated chrysin in gastric cancer cells. Artificial Cells, Nanomedicine, and Biotechnology. 2017; 45(6): 1201-1206.

5. Sadeghi, S., Haghighi, M., and Estifaee, P. Methanol to clean gasoline over nanostructured CuO–ZnO/HZSM-5 catalyst: Influence of conventional and ultrasound assisted co-impregnation synthesis on catalytic properties and performance. Journal of Natural Gas Science and Engineering. 2015; 24: 302-310.

6. Montazeri, M., Pilehvar-Soltanahmadi, Y., Mohaghegh, M., Panahi, A., Khodi, S., Zarghami, N., and Sadeghizadeh, M. (). Antiproliferative and apoptotic effect of dendrosomal curcumin nanoformulation in P53 mutant and wide-type cancer cell lines. Anti-Cancer Agents in Medicinal Chemistry-Anti-Cancer Agents). 2017; 17(5): 662-673.

7. Firouzi-Amandi, A., Dadashpour, M., Nouri, M., Zarghami, N., Serati-Nouri, H., Jafari-Gharabaghlou, D., and Pilehvar-Soltanahmadi, Y. Chrysin-nanoencapsulated PLGA-PEG for macrophage repolarization: Possible application in tissue regeneration. Biomedicine and Pharmacotherapy. 2018; 105: 773-780.

8. Farajzadeh, R., Zarghami, N., Serati-Nouri, H., Momeni-Javid, Z., Farajzadeh, T., Jalilzadeh-Tabrizi, S., and Pilehvar-Soltanahmadi, Y. Macrophage repolarization using CD44-targeting hyaluronic acid–polylactide nanoparticles containing curcumin. Artificial Cells, Nanomedicine, and Biotechnology. 2018; 46(8): 2013-2021.

9. Abedi-Gaballu, F., Dehghan, G., Ghaffari, M., Yekta, R., Abbaspour-Ravasjani, S., Baradaran, B., and Hamblin, M. R. PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy. Applied Materials Today. 2018; 12: 177-190.

10. Javidfar, S., Pilehvar-Soltanahmadi, Y., Farajzadeh, R., Lotfi-Attari, J., Shafiei-Irannejad, V., Hashemi, M., and Zarghami, N. (). The inhibitory effects of nano-encapsulated metformin on growth and hTERT expression in breast cancer cells. Journal of Drug Delivery Science and Technology. 2018; 43: 19-26.

11. Zhang, Y., Yan, W., Sun, Z., Pan, C., Mi, X., Zhao, G., and Gao, J. Fabrication of porous zeolite/chitosan monoliths and their applications for drug release and metal ions adsorption. Carbohydrate Polymers. 2015; 117: 657-665.

12. Rimoli, M. G., Rabaioli, M. R., Melisi, D., Curcio, A., Mondello, S., Mirabelli, R., and Abignente, E. Synthetic zeolites as a new tool for drug delivery. Journal of Biomedical Materials Research Part A. 2008; 87(1): 156-164.

13. Kang, J., Liu, H., Zheng, Y. M., Qu, J., and Chen, J. P. Application of nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, UV–Visible spectroscopy and kinetic modeling for elucidation of adsorption chemistry in uptake of tetracycline by zeolite beta. Journal of Colloid and Interface Science. 2011; 354(1): 261-267.

14. Mavrodinova, V., Popova, M., Yoncheva, K., Mihály, J., and Szegedi, Á. Solid-state encapsulation of Ag and sulfadiazine on zeolite Y carrier. Journal of Colloid and Interface Science. 2015; 458: 32-38.

15. Paradee, N., and Sirivat, A. Encapsulation of folic acid in zeolite y for controlled release via electric field. Molecular Pharmaceutics. 2016; 13(1): 155-162.

16. Krajišnik, D., Daković, A., Malenović, A., Kragović, M., and Milić, J. Ibuprofen sorption and release by modified natural zeolites as prospective drug carriers. Clay Minerals. 2015; 50(1): 11-22.

17. Talaei, S., Mellatyar, H., Pilehvar-Soltanahmadi, Y., Asadi, A., Akbarzadeh, A., and Zarghami, N. 17-Allylamino-17-demethoxygeldanamycin loaded PCL/PEG nanofibrous scaffold for effective growth inhibition of T47D breast cancer cells. Journal of Drug Delivery Science and Technology. 2019; 49: 162-168.

18. Mohseni-Bandpi, A., Al-Musawi, T. J., Ghahramani, E., Zarrabi, M., Mohebi, S., and Vahed, S. A. Improvement of zeolite adsorption capacity for cephalexin by coating with magnetic Fe3O4 nanoparticles. Journal of Molecular Liquids. 2016; 218: 615-624.

19. Abedi-Gaballu, F., Dehghan, G., Ghaffari, M., Yekta, R., Abbaspour-Ravasjani, S., Baradaran, B., and Hamblin, M. R. PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy. Applied Materials Today. 2018; 12:177-190.

20. Fatouros, D. G., Douroumis, D., Nikolakis, V., Ntais, S., Moschovi, A. M., Trivedi, V., and Cox, P. A. In vitro and in silico investigations of drug delivery via zeolite BEA. Journal of Materials Chemistry. 2011; 21(21): 7789-7794.

21. Sağir, T., Huysal, M., Durmus, Z., Kurt, B. Z., Senel, M., and Isık, S. Preparation and in vitro evaluation of 5-flourouracil loaded magnetite–zeolite nanocomposite (5-FU-MZNC) for cancer drug delivery applications. Biomedicine and Pharmacotherapy. 2016; 77: 182-190.

22. Bacakova, L., Vandrovcova, M., Kopova, I., and Jirka, I. Applications of zeolites in biotechnology and medicine–a review. Biomaterials Science. 2018; 6(5): 974-989.

23. Kumar, J., and Melo, J. S. Overview on biosensors for detection of organophosphate pesticides. Curr. Trends Biomed. Eng. Biosci, 2017; 5: 555-663.

24. Pan, Y., Zhan, S., and Xia, F. Zeolitic imidazolate framework-based biosensor for detection of HIV-1 DNA. Analytical Biochemistry. 2018; 546: 5-9.

25. Narang, J., Malhotra, N., Singhal, C., Mathur, A., Chakraborty, D., Anil, A., and Pundir, C. S. Point of care with micro fluidic paper based device integrated with nano zeolite–graphene oxide nanoflakes for electrochemical sensing of ketamine. Biosensors and Bioelectronics. 2017; 88: 249-257.

26. Kaur, B., and Srivastava, R. A polyaniline–zeolite nanocomposite material based acetylcholinesterase biosensor for the sensitive detection of acetylcholine and organophosphates. New Journal of Chemistry. 2015; 39(9): 6899-6906.

27. Zhang, X., Sun, J., Liu, J., Xu, H., Dong, B., Sun, X., and Song, H. Label-free electrochemical immunosensor based on conductive Ag contained EMT-style nano-zeolites and the application for α-fetoprotein detection. Sensors and Actuators B: Chemical, 2018; 255: 2919-2926.

28. Ninan, N., Muthiah, M., Yahaya, N. A. B., Park, I. K., Elain, A., Wong, T. W., and Grohens, Y. Antibacterial and wound healing analysis of gelatin/zeolite scaffolds. Colloids and Surfaces B: Biointerfaces. 2014; 115: 244-252.

29. Neidrauer, M., Ercan, U. K., Bhattacharyya, A., Samuels, J., Sedlak, J., Trikha, R., and Joshi, S. G. Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites. Journal of Medical Microbiology. 2014; 63(2): 203-209.

30. L. Naves, L. Almeida, World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2015; 9: 242–246.

31. Davarpanah Jazi, R., Rafienia, M., Salehi Rozve, H., Karamian, E., and Sattary, M. Fabrication and characterization of electrospun poly lactic-co-glycolic acid/zeolite nanocomposite scaffolds using bone tissue engineering. Journal of Bioactive and Compatible Polymers. 2018; 33(1): 63-78.

32. Mehrasa, M., Anarkoli, A. O., Rafienia, M., Ghasemi, N., Davary, N., Bonakdar, S., ... and Salamat, M. R. Incorporation of zeolite and silica nanoparticles into electrospun PVA/collagen nanofibrous scaffolds: the influence on the physical, chemical properties and cell behavior. International Journal of Polymeric Materials and Polymeric Biomaterials. 2016; 65(9): 457-465.

33. Thomassen, L. C., Napierska, D., Dinsdale, D., Lievens, N., Jammaer, J., Lison, D., and Martens, J. A. Investigation of the cytotoxicity of nanozeolites A and Y. Nanotoxicology. 2012; 6(5): 472-485.

34. Ferreira, L., Guedes, J. F., Almeida-Aguiar, C., Fonseca, A. M., and Neves, I. C. Microbial growth inhibition caused by Zn/Ag-Y zeolite materials with different amounts of silver. Colloids and Surfaces B: Biointerfaces. 2016; 142: 141-147.

35. Hrenovic, J., Milenkovic, J., Ivankovic, T., and Rajic, N. Antibacterial activity of heavy metal-loaded natural zeolite. Journal of Hazardous Materials. 2012; 201: 260-264.

36. Ferreira, L., Almeida-Aguiar, C., Parpot, P., Fonseca, A. M., and Neves, I. C. Preparation and assessment of antimicrobial properties of bimetallic materials based on NaY zeolite. RSC Advances. 2015; 5(47): 37188-37195.

37. Yu, L., Gong, J., Zeng, C., and Zhang, L. Preparation of zeolite-A/chitosan hybrid composites and their bioactivities and antimicrobial activities. Materials Science and Engineering: C. 2013; 33(7): 3652-3660.

38. Chen, S., Popovich, J., Iannuzo, N., Haydel, S. E., and Seo, D. K. Silver-ion-exchanged nanostructured zeolite X as antibacterial agent with superior ion release kinetics and efficacy against methicillin-resistant Staphylococcus aureus. ACS applied materials and interfaces. 2017; 9(45): 39271-39282.

39. Zhou, Y., Deng, Y., He, P., Dong, F., Xia, Y., and He, Y. Antibacterial zeolite with a high silver-loading content and excellent antibacterial performance. Rsc Advances. 2014; 4(10): 5283-5288.

40. Dong, B., Belkhair, S., Zaarour, M., Fisher, L., Verran, J., Tosheva, L., and Mintova, S. Silver confined within zeolite EMT nanoparticles: preparation and antibacterial properties. Nanoscale. 2014; 6(18): 10859-10864.

41. Chau, J. L. H., Lee, C. C., Yang, C. C., and Shih, H. H. Zeolite-coated steel fibers for friction materials applications. Proceedings of the Institution of Mechanical Engineers, Part L: Journal of Materials: Design and Applications. 2016; 230(1): 35-42.

42. Alipour, M., Aghazadeh, M., Akbarzadeh, A., Vafajoo, Z., Aghazadeh, Z., and Raeisdasteh Hokmabad, V. Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds. Artificial Cells, Nanomedicine, and Biotechnology. 2019; 47(1): 3431-3437.

Received on 14.11.2025 Revised on 06.12.2025

Accepted on 24.12.2025 Published on 31.01.2026

Available online from February 07, 2026

Asian J. Research Chem.2026; 19(1):38-42.

DOI: 10.52711/0974-4150.2026.00008

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